2.3 DNA: Merrifield solid phase synthesis of DNA

2.3 DNA: Merrifield solid phase synthesis of DNA

Merrifield solid phase synthesis of DNA

Comments

Post a Comment

Popular posts from this blog

1.3. Five membered heterocycles with Two heteroatoms: Imidazole, Oxazole & Thiazole

1.3. Five membered heterocycles with Two heteroatoms: a. Imidazole: Synthetic methods including synthesis from imidazolines, α-haloketones, Radiszewskii reaction. b. Oxazole: reaction between acid amides and α-halogenoketones eg. Acetamide and bromoacetone form 2,4-dimethyloxazole, Robinson–Gabriel synthesis by dehydration of 2-acylaminoketones, Reaction with Tosylmethyl isocyanide and aldehydes (The Van Leusen reaction) c. Thiazole: preparation α-chlorocarbonyl compound and thioacid amide– Hantzsch synthesis, Gabriel synthesis by reaction of a-Acylamino Ketones with Phosphorus Pentasulfide, Cook-Heilborn’s synthesis from a-Aminonitriles, Reactions of Imidazole, Thiazole, Oxazole with acids, Electrophilic Aromatic Substitution (EAS), nucleophilic aromatic substitution (NAS), oxidizing and reducing agents Imidazole, Oxazole & Thiazole
Read more

1.2 Five membered Heterocycles with One Heteroatom: Pyrrole, Furan & Thiophene

1.2 Five membered Heterocycles with One Heteroatom: a. Furan: Synthetic methods including synthesis using carbohydrates, Paal-Knorr synthesis b. Pyrrole: Synthetic methods including synthesis using furan, Knorr synthesis, Paal-Knorr synthesis, Hantzsch synthesis. c. Thiophene: Synthetic methods including synthesis using Paal-Knorr synthesis. Reactions of Furan, Pyrrole, and Thiophene: With acids, Electrophilic Aromatic Substitution (EAS), Nucleophilic Aromatic Substitution (NAS) reaction, oxidizing and reducing agents. Pyrrole, Furan & Thiophene
Read more

2.2 Biomolecules: Peptides

II. Peptides: Isoelectric point, synthesis of alpha amino acids (Strecker synthesis and amidomalonate and reductive amination of alpha keto acids), co-valent bonding in peptides, structure determination of peptides, sequencing of peptides (Edman synthesis, C-terminal residue determination- carboxy peptidase), partial hydrolysis of peptides using chemical (aq. Acids) and enzymatic methods (trypsin and chymotrypsin), synthesis of peptides – protection and deprotection of N and C-terminal amino acids, solution phase and solid phase (Merrifield) peptide synthesis. Peptides Isoelectric point synthesis of alpha amino acids John Mcmurry Peptides Solid Phase Peptide Synthesis : The Basics
Read more