2.2 Biomolecules: Peptides
II. Peptides:
Isoelectric point, synthesis of alpha amino acids (Strecker synthesis and amidomalonate and reductive amination of alpha keto acids), co-valent bonding in peptides, structure determination of peptides, sequencing of peptides (Edman synthesis, C-terminal residue determination- carboxy peptidase), partial hydrolysis of peptides using chemical (aq. Acids) and enzymatic methods (trypsin and chymotrypsin), synthesis of peptides – protection and deprotection of N and C-terminal amino acids, solution phase and solid phase (Merrifield) peptide synthesis.
Peptides
Isoelectric point
synthesis of alpha amino acids
John Mcmurry Peptides
Isoelectric point, synthesis of alpha amino acids (Strecker synthesis and amidomalonate and reductive amination of alpha keto acids), co-valent bonding in peptides, structure determination of peptides, sequencing of peptides (Edman synthesis, C-terminal residue determination- carboxy peptidase), partial hydrolysis of peptides using chemical (aq. Acids) and enzymatic methods (trypsin and chymotrypsin), synthesis of peptides – protection and deprotection of N and C-terminal amino acids, solution phase and solid phase (Merrifield) peptide synthesis.
Peptides
Isoelectric point
synthesis of alpha amino acids
John Mcmurry Peptides
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